Molecular characterization and functional expression of the human cardiac gap junction channel.

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Molecular characterization and functional expression of the human cardiac gap junction channel

Gap junctions permit the passage of ions and chemical mediators from cell to cell. To identify the molecular genetic basis for this coupling in the human heart, we have isolated clones from a human fetal cardiac cDNA library which encode the full-length human cardiac gap junction (HCGJ) mRNA. The predicted amino acid sequence is homologous to the rat cardiac gap junction protein, connexin43 (Be...

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Functional analysis of human cardiac gap junction channel mutants.

The connexins form a family of membrane spanning proteins that assemble into gap junction channels. The biophysical properties of these channels are dependent upon the constituent connexin isoform. To begin identifying the molecular basis for gap junction channel behavior in the human heart, a tissue that expresses connexin43, we used site-directed mutagenesis to generate mutant cDNAs of human ...

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Cardiac gap junction channels: modulation of expression and channel properties.

In the heart, intercellular gap junction channels constructed from connexin molecules are crucial for conduction of the electric impulse. Cardiomyocytes can be interconnected by channels composed of three types of connexin proteins: Cx40, Cx43 or Cx45. In mammalian hearts, these three isoforms are regionally differently expressed and even between the species differences exist. Each of these cha...

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Bovine connexin44, a lens gap junction protein: molecular cloning, immunologic characterization, and functional expression.

PURPOSE To identify, clone molecularly, characterize immunochemically, and express functionally a bovine lens gap junction protein (connexin). METHODS The methods used were polymerase chain reaction, genomic cloning, RNA and DNA blotting, bacterial expression of a fusion protein, immunoblotting, alkaline phosphatase treatment, Xenopus oocyte expression, and voltage clamp technique. RESULTS ...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1990

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.111.2.589